A federal judge in Alabama on Tuesday refused to block the Biden administration from enforcing new anti-discrimination protections for LGBTQ students in four Republican-led states, breaking with six other judges who have said the rules are invalid.

U.S. District Judge Annemarie Axon in Birmingham in a 122-page ruling, opens new tab rejected various arguments that the four states led by Alabama made in challenging U.S. Department of Education regulations that say a federal law barring sex discrimination in education extends to gender identity.

The regulations also bar harassment against LGBTQ students, such as refusing to use a transgender student’s preferred pronouns, and changes the procedures schools must use in investigating accusations of student misconduct.

Axon, an appointee of Republican former President Donald Trump, said the claims by the states and several conservative groups were conclusory and not backed by court precedent.

“Although Plaintiffs may dislike the Department’s rules, they have failed to show a substantial likelihood of success in proving the Department’s rulemaking was unreasonable or not reasonably explained,” Axon wrote.